Crohn's Disease Forum » Parents of Kids with IBD » Fecal caloprotectin home storage temp - per study - keep it COOL


04-01-2019, 08:45 PM   #1
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Fecal caloprotectin home storage temp - per study - keep it COOL

Calprotectin instability may lead to undertreatment in children with IBD
Sjoukje-Marije Haisma1, Patrick Ferry van Rheenen1, Lucie Wagenmakers2, Anneke Muller Kobold2
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Abstract

Background Treatment decisions in children with inflammatory bowel disease (IBD) are increasingly based on longitudinal tracking of faecal calprotectin concentrations, but there is little known about the stability of this protein in stool.

Methods We stored aliquots of homogenised stool at room temperature and at 4C, and measured the calprotectin concentration for 6 consecutive days with three different assays. In addition, we assessed calprotectin stability in assay-specific extraction buffers kept at room temperature.

Results After 6 days of storage at room temperature, mean percentage change from baseline calprotectin concentrations in stool and extraction buffer was 35% and 46%, respectively. The stability of calprotectin was significantly better preserved in samples stored at 4C (p=0.0066 and 0.0011, respectively).

Conclusions Calprotectin is not stable at room temperature. Children with IBD and their caretakers may be falsely reassured by low calprotectin values. The best advisable standard for preanalytical calprotectin handling is refrigeration of the stool sample until delivery at the hospital laboratory.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See:

From
https://adc.bmj.com/content/early/20...ld-2018-316584




Calprotectin instability may lead to undertreatment in children with IBD
Sjoukje-Marije Haisma1, Patrick Ferry van Rheenen1, Lucie Wagenmakers2, Anneke Muller Kobold2
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04-01-2019, 10:24 PM   #2
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