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http://www.sciencedirect.com/science/article/pii/S1043466612007910
Antibodies recognizing specific Mycobacterium avium subsp. paratuberculosis's MAP3738c protein in type 1 diabetes mellitus children are associated with serum Th1 (CXCL10) chemokine.
Cossu A, Ferrannini E, Fallahi P, Antonelli A, Sechi LA.
2012 Dec 18
Department of Biomedical Sciences, University of Sassari, Italy
Recently Mycobacterium avium subsp. paratuberculosis (Map) was associated to type 1 diabetes mellitus (T1DM). In this study we investigated for Map presence in children affected by T1DM compared to healthy children. A pool of 212 sera from T1DM children at onset was compared to sera from 57 healthy children for humoral immune response towards the Map specific protein MAP3738c by ELISA. Serum concentrations of CXCL10 (pro-Th1) and CCL2 (pro-Th2) chemokines were also measured in both sera pool. Results showed that T1DM children had a stronger seropositivity towards MAP3738c protein compared to healthy children. Data highlighted also the correlation between serum activity of T1DM patients towards the specific protein of Map and the increase of CXCL10 concentration if compared to non-diabetic subjects. In conclusion, an immune response to Map in T1DM patients at onset was observed and this may indicate a role of the bacterium in triggering or precipitating the disease.
Fig. 1. (A) Evaluation of immunoreactivity against MAP3738c recombinant protein in sera from 212 children with type I diabetes mellitus (red) and 57 healthy children (blue). Data are shown as values of OD405 nm for each serum in performed ELISA. The average value for each population is indicated by the bolded horizontal line. (B) Dot-plot for CCL2 concentration in sera from healthy children (blue) and T1DM children (red). The horizontal bar indicates the average CCL2 concentration within the group. (C) Dot-plot for CXCL10 concentration in sera from healthy children (blue) and T1DM children (red). The horizontal bar indicates the average CXCL10 concentration within the group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Antibodies recognizing specific Mycobacterium avium subsp. paratuberculosis's MAP3738c protein in type 1 diabetes mellitus children are associated with serum Th1 (CXCL10) chemokine.
Cossu A, Ferrannini E, Fallahi P, Antonelli A, Sechi LA.
2012 Dec 18
Department of Biomedical Sciences, University of Sassari, Italy
Recently Mycobacterium avium subsp. paratuberculosis (Map) was associated to type 1 diabetes mellitus (T1DM). In this study we investigated for Map presence in children affected by T1DM compared to healthy children. A pool of 212 sera from T1DM children at onset was compared to sera from 57 healthy children for humoral immune response towards the Map specific protein MAP3738c by ELISA. Serum concentrations of CXCL10 (pro-Th1) and CCL2 (pro-Th2) chemokines were also measured in both sera pool. Results showed that T1DM children had a stronger seropositivity towards MAP3738c protein compared to healthy children. Data highlighted also the correlation between serum activity of T1DM patients towards the specific protein of Map and the increase of CXCL10 concentration if compared to non-diabetic subjects. In conclusion, an immune response to Map in T1DM patients at onset was observed and this may indicate a role of the bacterium in triggering or precipitating the disease.
Fig. 1. (A) Evaluation of immunoreactivity against MAP3738c recombinant protein in sera from 212 children with type I diabetes mellitus (red) and 57 healthy children (blue). Data are shown as values of OD405 nm for each serum in performed ELISA. The average value for each population is indicated by the bolded horizontal line. (B) Dot-plot for CCL2 concentration in sera from healthy children (blue) and T1DM children (red). The horizontal bar indicates the average CCL2 concentration within the group. (C) Dot-plot for CXCL10 concentration in sera from healthy children (blue) and T1DM children (red). The horizontal bar indicates the average CXCL10 concentration within the group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
