Influences of EN upon CEACAM6 expression by intestinal epithelial cells

[kiny]

http://ini.sagepub.com/content/early/2013/12/06/1753425913513309.long

Keenan JI, Hooper EM, Tyrer PC, Day AS

2013 Dec 10

Influences of enteral nutrition upon CEACAM6 expression by intestinal epithelial cells.

Exclusive enteral nutrition is established as an initial therapy to induce remission in active Crohn's disease (CD), especially in children, but the mechanisms of action of this therapy are yet to be fully defined. CEACAM6 protein is an adhesion molecule that is up-regulated in active CD and implicated in the attachment of adherent-invasive Escherichia coli (AIEC) to the gut epithelium. Using the Caco-2 human adenocarcinoma cell line, this study showed that the incubation of human cells with a polymeric formula (PF) resulted in a dose-dependent increase in the expression of CEACAM6, and that this effect was most noticeable on the cell surface. Further investigation revealed that PF doubled the release of CEACAM6 protein by Caco-2 cells exposed to PF, and that an increase in release of soluble CEACAM6 inversely correlated with the ability of AIEC to associate with the intestinal epithelial cells. Our findings suggest that the secretion of cell surface-associated proteins acting as releasable decoys may be an aspect of the gut's innate immune response to pathogenic bacteria that is strengthened by PF in the setting of CD.

 

[my little penguin]

Very cool
Thank you !

 

[Jane]

Kiny -

Can you translate the findings into plain lay person english for me?

"Our findings suggest that the secretion of cell surface-associated proteins acting as releasable decoys may be an aspect of the gut's innate immune response to pathogenic bacteria that is strengthened by PF in the setting of CD. "

Does this mean it is beneficial to use a polymeric forumula?

Thanks for sharing!

Jane

 

[kiny]

Kiny -

Can you translate the findings into plain lay person english for me?

"Our findings suggest that the secretion of cell surface-associated proteins acting as releasable decoys may be an aspect of the gut's innate immune response to pathogenic bacteria that is strengthened by PF in the setting of CD. "

Does this mean it is beneficial to use a polymeric forumula?

Thanks for sharing!

Jane

I don't want to say it is beneficial, I don't want to tell anyone what to do.

AIEC, as the name implies are adherent and invasive types of E Coli. They specifically look for ways to attach to cells, it is much easier for them to survive if they don't stay in the gut lumen but manage to attach themselves to the lining of the intestine, and they eventually penetrate the intestine, they do this by creating biofilms and by binding to certain cells. CEACAM6 is a receptor for those bacteria, it's overexpressed in people with crohn's disease.

People without CD sometimes have AIEC too, but they don't seem to be able to invade their intestine, they also have low CEACAM6 expression.

They're saying that the body is able to use soluble CEACAM6 which acts as a decoy attachment protein, and PF stimulates the amount of decoys available, so those bacteria aren't attaching themselves to the intestine.

The problem with AIEC is really it's ability to attach itself to the intestine and it's ability to invade the intestine, it's a really pathogenic form of E Coli, most E Coli found in people without Crohn's disease are quite harmless. In fact E Coli of the indigenous flora are probably helpful in stopping the spread of AIEC. Lumen bacteria are in a constant struggle to stay alive, and they can only do this by fighting for their spot.

so in English: they think the body is able to make decoys to limit the spread of AIEC, and they think EN formulas help the body accomplish this

 

[Jane]

Cool! Thank you for explaining to me.