Crohn's Disease May Be Differentiated Into 2 Distinct Biotypes Based on the Detection of Bacterial Genomic Sequences and Virulence Genes Wit

[kiny]

sent to me, ty

http://www.ncbi.nlm.nih.gov/pubmed/?term=Crohn%27s+Disease+May+Be+Differentiated+Into+2+Distinct+Biotypes+Based+on+the+Detection+of+Bacterial+Genomic+Sequences+and+Virulence+Genes+Within+Submucosal+Tissues.


Crohn's Disease May Be Differentiated Into 2 Distinct Biotypes Based on the Detection of Bacterial Genomic Sequences and Virulence Genes Within Submucosal Tissues.

Molecular Research (Mr. DNA), Shallowater, TX

Chiodini RJ, Dowd SE, Davis B, Galandiuk S, Chamberlin WM, Kuenstner JT, McCallum RW, Zhang J.

2013 Feb 18

OBJECTIVE::

To determine whether bacterial pathogens can be detected within the diseased submucosal tissues of patients with Crohn's disease by molecular techniques independent of cultural methods.

DESIGN:: We designed a quantitative polymerase chain reaction to detect 32 virulence genes and transposons within submucosal tissues of patients with Crohn's disease and controls and compared the microbiome of the submucosa with mucosal bacterial populations.

RESULTS:: Within submucosal tissues, the bacterial invasion/adherence genes eaeA and invA were detected in 43% of patients (P=0.01 and 0.008 vs. mucosa and controls, respectively) and the Mycobacterium-specific IS900 and 251F genes detected in 50% of patients (P=0.03 vs. mucosa and controls). These findings were mutually exclusive: invasion/adhesion genes and Mycobacterium-associated transposons were not detected in the same patient. Metagenomic sequencing and quantitative polymerase chain reaction results confirmed effective separation of the submucosal and mucosal microbiome and the existence of a submucosal bacterial population within diseased tissues.

CONCLUSIONS:: This study is the first to examine the microbial populations of submucosal tissues during intestinal disease and provide evidence of a distinct submucosal microbiome and biotypes within Crohn's disease. These data suggests that Crohn's disease may not be a single disease, but a spectrum that can be divided into distinct biotypes based on the presence of invasion/adherence genes or Mycobacterium-associated transposons. If corroborated by larger population studies, these findings could revolutionize the diagnosis, management, and treatment of Crohn's disease by the identification of patient biotypes and the application of targeted chemotherapeutic treatments that go beyond supportive in nature.

 

[kiny]

tissue from 14 people with crohn were used from people who had surgery
6 controls

the tissue was vortexed and washed in phosphate-buffered saline to avoid contamination, homogenized and centrifuged, DNA for testing was taken from the sediment

they used NRG 857C and LF82 to select AIEC genes


I can't tell how biased or unbiased this study is, but I know it gets tiring to see people readily detect antigen in submucosa and others find didly squat. One side is wrong here.

 

[rollinstone]

Would be awesome for them to further test this in larger numbers, or atleast compile results from various instances of the similar experiments

 

[kiny]

Yes, it needs to be done in a large number by a group with as little bias as possible, using the same methods and using transmural tissue. Currently these tests are all over the place, one is using PCR, the other immunostaining - culture etc, one is using miniscule biopsy tissue, the other is using submucosa. This isn't helping anyone, it just goes around and around and people hurting are people with crohn's disease.

 

[David]

Fascinating. Thanks Kiny!

 

[my little penguin]

Very cool!